Itakura M, Tsuchiya M, Yamashita K
Adv Exp Med Biol. 1986;195 Pt B:347-55. doi: 10.1007/978-1-4684-1248-2_55.
The labeling of hepatic purines with [14C]glycine increased in the regenerating rat liver 12 hours after a 70% hepatectomy and it reached the 2.4 folds higher peak 12 hours after the surgical operation in comparison to sham-operated control. These observations suggest that the rate of de novo biosynthesis increases in the regenerating rat liver to supply purine ribonucleotides to the cells. In the regenerating rat liver the activity of amidophosphoribosyltransferase (ATase) significantly increased and reached the 1.8 folds higher peak than control 18 hours after surgery. Hepatic 5-phosphoribosyl 1-pyrophosphate (PRPP) concentration significantly increased and reached the 3.0 folds higher peak 12 hours after surgery. Although the mean hepatic concentration of ATP and GTP showed a 13 and 15% decrease respectively in the regenerating rat liver 12 hours after surgery, they were counterbalanced by the increased concentrations of AMP and GMP by 13 and 44% respectively. These results suggest that the increased rate of de novo purine biosynthesis in the regenerating rat liver is mediated by the increased enzymatic activity of ATase and by the increased concentration of PRPP.
70%肝切除术后12小时，再生大鼠肝脏中用[14C]甘氨酸标记的肝嘌呤增加，与假手术对照组相比，术后12小时达到高2.4倍的峰值。这些观察结果表明，再生大鼠肝脏中从头生物合成速率增加，以向细胞供应嘌呤核糖核苷酸。在再生大鼠肝脏中，氨甲酰磷酸核糖转移酶（ATase）的活性显著增加，术后18小时达到比对照组高1.8倍的峰值。肝脏5-磷酸核糖-1-焦磷酸（PRPP）浓度显著增加，术后12小时达到高3.0倍的峰值。尽管术后12小时再生大鼠肝脏中ATP和GTP的平均肝脏浓度分别下降了13%和15%，但它们分别被AMP和GMP浓度增加13%和44%所抵消。这些结果表明，再生大鼠肝脏中从头嘌呤生物合成速率的增加是由ATase酶活性的增加和PRPP浓度的增加介导的。
