Itakura M, Maeda N, Tsuchiya M, Yamashita K
Am J Physiol. 1986 Nov;251(5 Pt 1):G585-90. doi: 10.1152/ajpgi.1986.251.5.G585.
In regenerating rat liver at 12 h after a 70% hepatectomy, [14C]glycine incorporation to hepatic acid-soluble purines increased by 2.4-fold with a 26% increase of the mean rapidly miscible glycine pool size and its comparable turnover rate. Amidophosphoribosyltransferase (EC 2.4.2.14, ATase) activity increased 1.8-fold at 18 h and 5-phosphoribosyl-1-pyrophosphate (PRPP) concentration increased 3.0-fold at 12 h after surgery. Decreases in ATP and GTP concentrations of 13 and 15%, respectively, in regenerating rat liver at 12 h after surgery were counterbalanced by increased AMP and GMP concentrations of 13 and 44%, respectively, with comparable inhibitory potentials on ATase. These results suggest than an increased rate of de novo purine synthesis in regenerating rat liver is mediated by the increased ATase activity and PRPP concentrations.
在70%肝切除术后12小时的再生大鼠肝脏中，[14C]甘氨酸掺入肝酸溶性嘌呤的量增加了2.4倍，同时平均快速可混溶甘氨酸池大小增加了26%，其周转速率相当。氨甲酰磷酸核糖转移酶（EC 2.4.2.14，ATase）活性在术后18小时增加了1.8倍，5-磷酸核糖-1-焦磷酸（PRPP）浓度在术后12小时增加了3.0倍。术后12小时再生大鼠肝脏中ATP和GTP浓度分别降低了13%和15%，但分别增加了13%和44%的AMP和GMP浓度对其进行了抵消，且对ATase具有相当的抑制潜力。这些结果表明，再生大鼠肝脏中嘌呤从头合成速率的增加是由ATase活性和PRPP浓度的增加介导的。
