血管内皮生长因子促进大鼠坐骨神经横断后周围神经再生。

Vascular endothelial growth factor promotes peripheral nerve regeneration after sciatic nerve transection in rat.

作者信息

Mohammadi Rahim, Ahsan Sima, Masoumi Masoume, Amini Keyvan

机构信息

Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia 571531177, Iran.

出版信息

Chin J Traumatol. 2013;16(6):323-9.

DOI:

PMID:24295576

Abstract

OBJECTIVE

To evaluate the local effect of vascular endothelial growth factor (VEGF) on transected sciatic nerve regeneration.

METHODS

Sixty male white Wistar rats were divided into four experimental groups randomly (n equal to 15). In transected group the left sciatic nerve was transected and the stump was fixed to adjacent muscle. In treatment group the defect was bridged using a silicone graft filled with 10 microlitre VEGF. In silicone group the graft was filled with phosphate-buffered saline. In sham-operated group the sciatic nerve was exposed and manipulated. Each group was subdivided into three subgroups with five animals in each and nerve fibers were studied 4, 8 and 12 weeks after operation.

RESULTS

Behavioral test, functional study of sciatic nerve, gastrocnemius muscle mass and morphometric indices confirmed a faster recovery of regenerated axons in VEGF group than in silicone group (P less than 0.05). In immunohistochemical assessment, reactions to S-100 in VEGF group were more positive than that in silicone group.

CONCLUSION

Local administration of VEGF will improve functional recovery and morphometric indices of sciatic nerve.

摘要

目的

评估血管内皮生长因子（VEGF）对坐骨神经横断后再生的局部作用。

方法

将60只雄性白色Wistar大鼠随机分为4个实验组（每组n = 15）。横断组切断左侧坐骨神经，将残端固定于邻近肌肉。治疗组用填充有10微升VEGF的硅胶移植物桥接缺损。硅胶组移植物填充磷酸盐缓冲盐水。假手术组暴露并操作坐骨神经。每组再分为3个亚组，每组5只动物，于术后4周、8周和12周研究神经纤维。