Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Nazloo Road, Urmia 57153 1177, Iran.
Injury. 2013 Oct;44(10):1295-301. doi: 10.1016/j.injury.2013.04.014. Epub 2013 Jun 6.
The objective was to assess the effect of topically administered insulin-like growth factor (IGF I) on peripheral nerve regeneration and functional recovery. Eighty male healthy white Wistar rats were divided into four experimental groups (n=20), randomly: in transected group (TC), the left sciatic nerve was transected and stumps were fixed in the adjacent muscle. In treatment group, defect was bridged using an inside-out artery graft (IOAG/IGF) filled with 10 μL IGF I (100 ng/kg). In artery graft group (IOAG), the graft was filled with phosphate-buffered saline alone. In sham-operated group (SHAM), sciatic nerve was exposed and manipulated. Each group was subdivided into five subgroups of five animals each and regenerated nerve fibres were studied 4, 8, 12 and 16 weeks after surgery. Behavioural testing, sciatic nerve functional study, gastrocnemius muscle mass and morphometric indices confirmed faster recovery of regenerated axons in IOAG/IGF than IOAG group (P<0.05). In immunohistochemistry, location of reactions to S-100 in IOAG/IGF was clearly more positive than that in IOAG group. When loaded in an artery graft, IGF I accelerated and improved functional recovery and morphometric indices of sciatic nerve.
目的是评估局部给予胰岛素样生长因子（IGF I）对周围神经再生和功能恢复的影响。将 80 只雄性健康白色 Wistar 大鼠随机分为 4 个实验组（n=20）：在横断组（TC）中，左侧坐骨神经被横断，残端固定在相邻的肌肉中。在治疗组中，使用充满 10 μL IGF I（100 ng/kg）的外翻动脉移植物（IOAG/IGF）桥接缺损。在动脉移植物组（IOAG）中，移植物中仅填充磷酸盐缓冲盐水。在假手术组（SHAM）中，暴露并操作坐骨神经。每组进一步分为 5 个每组 5 只动物的亚组，在手术后 4、8、12 和 16 周研究再生神经纤维。行为测试、坐骨神经功能研究、腓肠肌质量和形态计量学指标证实，在 IOAG/IGF 组中，再生轴突的恢复速度明显快于 IOAG 组（P<0.05）。在免疫组织化学中，IOAG/IGF 中 S-100 反应的定位明显比 IOAG 组更阳性。当 IGF I 加载到动脉移植物中时，它可以加速和改善坐骨神经的功能恢复和形态计量学指标。
