Watanabe Ken, Iwashima Yasuki, Akutsu Tomoko, Sekiguchi Kazumasa, Sakurada Koichi
National Research Institute of Police Science, Chiba 277-0882, Japan.
National Research Institute of Police Science, Chiba 277-0882, Japan.
Leg Med (Tokyo). 2014 Jan;16(1):56-9. doi: 10.1016/j.legalmed.2013.11.002. Epub 2013 Nov 19.
Body fluid identification and individual identification are an important series of tests in usual criminal investigations. Recent reports have demonstrated a new approach using DNA/RNA co-extraction methods in which RNA for body fluid identification and DNA for short tandem repeat (STR) typing are extracted simultaneously from the same sample. This study evaluated a standard co-extraction kit, the AllPrep® DNA/RNA Mini Kit, in order to demonstrate the availability of the co-extraction procedure for those real-time polymerase chain reaction-based body fluid identification methods that we have validated previously. We demonstrated that the use of the Allprep Kit, for which we adjusted the lysis temperature to 56°C to improve extraction efficiency, can simultaneously extract sufficient RNA and DNA for body fluid identification and STR analysis; however, a longer incubation at a high temperature slightly affected the ΔCt value of each target gene and appeared to be not as effective for DNA extraction from old stains as from 1-day-old stains. This method is promising for future forensic investigations because the use of this kit can reduce sample consumption for body fluid identification and DNA typing.
体液鉴定和个体识别是常规刑事调查中的一系列重要检测。最近的报告展示了一种使用DNA/RNA共提取方法的新途径，其中用于体液鉴定的RNA和用于短串联重复序列（STR）分型的DNA是从同一样本中同时提取的。本研究评估了一种标准共提取试剂盒——AllPrep® DNA/RNA Mini试剂盒，以证明这种共提取程序对于我们之前已验证的基于实时聚合酶链反应的体液鉴定方法的可用性。我们证明，使用Allprep试剂盒（我们将裂解温度调整为56°C以提高提取效率）能够同时提取足够的RNA和DNA用于体液鉴定和STR分析；然而，在高温下较长时间的孵育会对每个靶基因的ΔCt值产生轻微影响，并且对于从陈旧污渍中提取DNA似乎不如从1日龄污渍中提取有效。这种方法对未来的法医调查很有前景，因为使用该试剂盒可以减少体液鉴定和DNA分型的样本消耗。
