Gephardt G N, Tubbs R R, Popowniak K L, McMahon J T
Arch Pathol Lab Med. 1986 Oct;110(10):902-5.
To evaluate the deposition of immunoglobulins and complement and their relationship to sclerotic and nonsclerotic glomerular segments, immunoperoxidase without periodic acid-Schiff counterstain (IMP) and with periodic acid-Schiff counterstain (IMPAS) for IgG, IgA, IgM, and C3 was performed on cryostat-frozen sections using the direct method, along with routine light microscopy and electron microscopy, in a series of 20 renal biopsy specimens from 20 patients with the final diagnosis of focal and segmental glomerulosclerosis. Neither diffuse mesangial nor diffuse glomerular basement membrane deposits were detected by IMP, IMPAS, or electron microscopy. In 18 biopsy specimens, IMPAS demonstrated focal and segmental granular to globular deposits of IgM and/or C3 in sclerotic glomerular segments. In eight biopsy specimens, small granular deposits of IgM and/or C3 deposits were identified in optically normal glomeruli, suggesting that these deposits may precede segmental sclerosis.
为评估免疫球蛋白和补体的沉积情况及其与硬化性和非硬化性肾小球节段的关系，采用直接法，对20例最终诊断为局灶节段性肾小球硬化患者的20份肾活检标本的低温冷冻切片进行了不伴过碘酸-希夫复染的免疫过氧化物酶法（IMP）以及伴过碘酸-希夫复染的免疫过氧化物酶法（IMPAS）检测IgG、IgA、IgM和C3，同时进行常规光学显微镜和电子显微镜检查。IMP、IMPAS或电子显微镜均未检测到弥漫性系膜或弥漫性肾小球基底膜沉积。在18份活检标本中，IMPAS显示硬化性肾小球节段有IgM和/或C3的局灶节段性颗粒状至球状沉积。在8份活检标本中，在光学显微镜下正常的肾小球中发现了IgM和/或C3的小颗粒状沉积，提示这些沉积可能先于节段性硬化出现。
