Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas, Eduardo Cabello, 6, 36208 Vigo, Spain.
Interdisciplinary Center of Marine and Environmental Research, University of Porto, Rua dos Bragas 289, 4050-123 Porto, Portugal; Institute of Biomedical Sciences Abel Salazar, University of Porto, Rua Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal.
Dev Comp Immunol. 2014 May;44(1):50-8. doi: 10.1016/j.dci.2013.11.013. Epub 2013 Dec 1.
The hemocytes of Octopus vulgaris were morphologically and functionally characterized. Light and electron microscopy (TEM and SEM), and flow cytometry analyses revealed the existence of two hemocyte populations. Large granulocytes showed U-shaped nucleus, a mean of 11.6 μm±1.2 in diameter with basophilic granules, polysaccharide and lysosomic deposits in the cytoplasm. Small granulocytes measured a mean of 8.1 μm±0.7 in diameter, and have a round nucleus occupying almost the entire cell and few or not granules in the cytoplasm. Flow cytometry analysis showed that large granulocytes are the principal cells that develop phagocytosis of latex beads (rising up to 56%) and ROS after zymosan stimulation. Zymosan induced the highest production of both ROS and NO. This study is the first tread towards understanding the O. vulgaris immune system by applying new tools to provide a most comprehensive morpho-functional study of their hemocytes.
章鱼的血细胞在形态和功能上具有特征性。光镜和电子显微镜（透射电镜和扫描电镜）以及流式细胞术分析显示存在两种血细胞群体。大型粒细胞呈 U 形核，平均直径为 11.6μm±1.2μm，细胞质中含有嗜碱性颗粒、多糖和溶酶体沉积物。小型粒细胞平均直径为 8.1μm±0.7μm，圆形核几乎占据整个细胞，细胞质中颗粒较少或没有。流式细胞术分析表明，大型粒细胞是主要的细胞，能够吞噬乳胶珠（上升高达 56%）和几丁质刺激后的 ROS。几丁质诱导产生了最高水平的 ROS 和 NO。本研究首次应用新工具研究章鱼的免疫系统，对其血细胞进行了最全面的形态和功能研究。
