Schmid A, Barhanin J, Mourre C, Coppola T, Borsotto M, Lazdunski M
Biochem Biophys Res Commun. 1986 Sep 30;139(3):996-1002. doi: 10.1016/s0006-291x(86)80276-5.
Rabbit brain synaptosomes bind the 1,4-dihydropyridine derivative (+)[3H]-PN 200-110 with an equilibrium dissociation constant of 0.04 nM and a maximal binding capacity of 400 fmol/mg of protein. Using polyclonal antibodies raised against the different components of the skeletal muscle 1,4-dihydropyridine receptor, we have demonstrated that the brain and muscle receptors share the same subunit composition comprising a large polypeptide chain of Mr 140,000 associated by disulfide bridges with a smaller peptide of Mr 32,000. These antibodies have been used in immunofluorescence staining of brain sections. They reveal a distribution of the Ca2+ channel protein similar to that of 1,4-dihydropyridine binding sites with (+)[3H]PN 200-110 by the autoradiographic technique.
兔脑突触体结合1,4 - 二氢吡啶衍生物（+）[³H] - PN 200 - 110，其平衡解离常数为0.04 nM，最大结合容量为400 fmol/mg蛋白质。利用针对骨骼肌1,4 - 二氢吡啶受体不同组分产生的多克隆抗体，我们已证明脑和肌肉受体具有相同的亚基组成，包括一条Mr 140,000的大多肽链，通过二硫键与一条Mr 32,000的较小肽链相连。这些抗体已用于脑切片的免疫荧光染色。通过放射自显影技术，它们揭示的Ca²⁺通道蛋白分布与用（+）[³H]PN 200 - 110显示的1,4 - 二氢吡啶结合位点的分布相似。
