Puchtler H, Waldrop F S, Meloan S N
Appl Pathol. 1985;3(1-2):5-17.
Traditional technics for amyloid are not always dependable. Therefore, several reactions, which do not require differentiation, were developed in this laboratory. This review describes technics that proved suitable for diagnostic pathology, including polarization and fluorescence microscopy. Effects of fixation on the reactivity of amyloid are also considered. The chemical mechanism of the alkaline Congo red, Mesitol WLS-Congo red and Phorwhite BBU reaction, and of a modified thioflavine T stain are reviewed briefly. Problems encountered with other methods, which cannot be recommended for diagnostic pathology, are outlined.
传统的淀粉样蛋白检测技术并不总是可靠的。因此，本实验室开发了几种无需鉴别过程的反应方法。本综述介绍了已证明适用于诊断病理学的技术，包括偏振光显微镜和荧光显微镜技术。同时也考虑了固定对淀粉样蛋白反应性的影响。简要回顾了碱性刚果红、间四甲苯基白光刚果红和荧光增白剂BBU反应以及改良硫黄素T染色的化学机制。概述了其他不推荐用于诊断病理学的方法所遇到的问题。
