Chemnitz J, Folkersen J, Teisner B, Sinosich M J, Tornehave D, Westergaard J G, Bolton A E, Grudzinskas J G
Br J Obstet Gynaecol. 1986 Sep;93(9):916-23. doi: 10.1111/j.1471-0528.1986.tb08008.x.
Four antibody preparations against pregnancy-associated plasma protein (PAPP-A) were compared in order to find an explanation for the contradictory results published on tissue localization, clinical usefulness and biological function of PAPP-A. One of the preparations studied was a rabbit anti-PAPP-A antiserum which has been offered for general scientific use (Bischof et al. 1979). Only the IgG fraction of anti-PAPP-A antisera which appeared to be monospecific and had been further absorbed with fetal connective tissue gave specific uniform staining of the cytoplasm of the syncytiotrophoblast exclusively. Circulating PAPP-A could not be detected by RIA employing this IgG preparation in the non-pregnant state, or before 18 days after conception. Circulating PAPP-A could be detected in all seven pregnant women studied within 4 weeks after conception. Identical results were obtained with a commercially available IgG fraction against PAPP-A.
对四种抗妊娠相关血浆蛋白（PAPP-A）的抗体制剂进行了比较，以便为关于PAPP-A的组织定位、临床应用和生物学功能所发表的相互矛盾的结果找到解释。所研究的制剂之一是一种兔抗PAPP-A抗血清，它已被提供用于一般科学用途（比绍夫等人，1979年）。只有抗PAPP-A抗血清的IgG部分似乎是单特异性的，并且已用胎儿结缔组织进一步吸收，仅对合体滋养层细胞质产生特异性均匀染色。在非妊娠状态或受孕后18天之前，使用这种IgG制剂通过放射免疫分析无法检测到循环中的PAPP-A。在受孕后4周内，对所有七名孕妇进行研究时均检测到了循环中的PAPP-A。使用市售的抗PAPP-A的IgG部分也获得了相同的结果。
