一种使用单克隆抗体的人妊娠相关血浆蛋白A（PAPP-A）双位点免疫放射分析方法。

A two-site immunoradiometric assay for human pregnancy-associated plasma protein A (PAPP-A) using monoclonal antibodies.

作者信息

Mowles E A, Pinto-Furtado L G, Bolton A E

出版信息

J Immunol Methods. 1986 Dec 4;95(1):129-33. doi: 10.1016/0022-1759(86)90327-3.

Abstract

A rapid, sensitive immunoradiometric assay has been developed for human pregnancy-associated plasma protein A (PAPP-A) using a purified mouse monoclonal antibody as the tracer and a rabbit polyclonal antibody to this protein in the solid-phase antibody preparation. The assay showed no measurable cross-reaction (less than 0.1%) against a range of purified human placental proteins, and a good correlation with a previously described radioimmunoassay procedure when tested on samples taken throughout normal human pregnancies. No PAPP-A-like immunological activity could be detected in sera from non-pregnant women, confirming the absence of this protein from the circulation outside pregnancy.

摘要

已开发出一种快速、灵敏的免疫放射分析方法，用于检测人妊娠相关血浆蛋白A（PAPP-A）。该方法使用纯化的小鼠单克隆抗体作为示踪剂，以及针对该蛋白的兔多克隆抗体用于固相抗体制备。该分析方法对一系列纯化的人胎盘蛋白未显示出可测量的交叉反应（小于0.1%），并且在对正常妊娠全过程采集的样本进行检测时，与先前描述的放射免疫分析方法具有良好的相关性。在未怀孕女性的血清中未检测到PAPP-A样免疫活性，证实妊娠外循环中不存在这种蛋白。