Dondoni L, Scarparo R K, Kantarci A, Van Dyke T E, Figueiredo J A P, Batista E L
Pontificia Universidade Catolica do Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.
Int Endod J. 2014 Sep;47(9):827-34. doi: 10.1111/iej.12224. Epub 2014 Jan 13.
To evaluate the effects of topical Resolvin E1 (RvE1) application on infected dental pulps.
Forty-two male Wistar rats (n = 6 per three groups/and two time periods) were used. To induce inflammation, pulps in mandibular right first molars were accessed and then left exposed to the oral environment for 24 h. After this period, topical medication with a corticosteroid/antibiotic blend, or RvE1, or its vehicle (Ethanol 0.1%) was directly applied onto the pulp tissue and teeth were restored with silver amalgam. The effects of the protocols were evaluated histologically and compared with control pulps not exposed to the oral environment. The inflammatory changes after 24 and 72 h were assessed through a scoring method and analysed using the Kruskal-Wallis test followed by Dunn's. Differences were considered significant if P < 0.05 (CI = 95%).
Ethanol and corticosteroid/antibiotic treatment were not effective in arresting severe inflammatory alterations of exposed pulps at 24 and 72 h (P < 0.05, CI = 95%). At both time periods, RvE1 treatment led to a reduction of tissue cellularity and extent of inflammation, whose changes were not different from control pulps (P > 0.05, CI = 95%).
A protective role for RvE1 in pulp inflammation was observed even in the presence of contamination, suggesting that it may be a candidate for a novel therapeutic strategy for conservative dental pulp treatment.
评估局部应用消退素E1（RvE1）对感染牙髓的影响。
使用42只雄性Wistar大鼠（每三个组/两个时间段每组n = 6只）。为诱导炎症，打开下颌右侧第一磨牙的牙髓，然后使其暴露于口腔环境24小时。在此期间后，将含有皮质类固醇/抗生素混合物、RvE1或其赋形剂（0.1%乙醇）的局部药物直接应用于牙髓组织，并用银汞合金修复牙齿。通过组织学评估方案的效果，并与未暴露于口腔环境的对照牙髓进行比较。通过评分方法评估24小时和72小时后的炎症变化，并使用Kruskal-Wallis检验和随后的Dunn检验进行分析。如果P < 0.05（CI = 95%），则认为差异具有统计学意义。
乙醇和皮质类固醇/抗生素治疗在24小时和72小时时未能有效阻止暴露牙髓的严重炎症改变（P < 0.05，CI = 95%）。在两个时间段，RvE1治疗均导致组织细胞数量减少和炎症程度降低，其变化与对照牙髓无差异（P > 0.05，CI = 95%）。
即使存在污染，也观察到RvE1在牙髓炎症中具有保护作用，这表明它可能是保守牙髓治疗新策略的候选药物。
