Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan.
Biochem Biophys Res Commun. 2010 Sep 10;400(1):128-33. doi: 10.1016/j.bbrc.2010.08.025. Epub 2010 Aug 12.
Resolvin E1 (RvE1) is an anti-inflammatory lipid mediator derived from the omega-3 fatty acid eicosapentaenoic acid (EPA), and strongly acts in the resolution of inflammation. We previously reported that RvE1 dampens airway inflammation and hyperresponsiveness in a murine model of asthma. In the present study, to elucidate the effects of RvE1 on the development of asthmatic airway inflammation, we investigated whether RvE1 acts on different phases of an OVA-sensitized and -challenged mouse model of asthma. RvE1 treatments at the time of either OVA sensitization or at the time of OVA challenge were investigated and compared with RvE1 treatments at the time of both OVA sensitization and challenge. After RvE1 was administered to mice intraperitoneally at the time of both OVA sensitization and challenge, there were decreases in airway eosinophil and lymphocyte recruitment, as well as a reduction in Th2 cytokine and airway hyperresponsiveness. RvE1 treatment at the time of either OVA sensitization or challenge also improved AHR and airway inflammation. Our results suggest that RvE1 acts on several phases of asthmatic inflammation and may have anti-inflammatory effects on various cell types.
解析 E1（RvE1）是一种抗炎脂质介质，来源于 ω-3 脂肪酸二十碳五烯酸（EPA），可强烈促进炎症消退。我们之前报道过，RvE1 可减轻哮喘小鼠模型中的气道炎症和气道高反应性。在本研究中，为阐明 RvE1 对哮喘气道炎症发展的影响，我们研究了 RvE1 是否作用于 OVA 致敏和激发小鼠哮喘模型的不同阶段。研究了在 OVA 致敏或 OVA 激发时给予 RvE1 治疗，并与在 OVA 致敏和激发时同时给予 RvE1 治疗进行了比较。在 OVA 致敏和激发时同时给予腹腔内 RvE1 后，气道嗜酸性粒细胞和淋巴细胞募集减少，Th2 细胞因子减少，气道高反应性降低。在 OVA 致敏或激发时给予 RvE1 治疗也可改善 AHR 和气道炎症。我们的结果表明，RvE1 作用于哮喘炎症的多个阶段，可能对多种细胞类型具有抗炎作用。
