Mestiri Imen, Norre Frédéric, Gallego Maria E, White Charles I
Génétique, Reproduction et Développement, UMR CNRS 6293, Clermont Université, INSERM U1103, UFR Sciences et Technologies, Université Blaise Pascal, 24 avenue des Landais, BP80026, 63171, Aubière Cedex, France.
Plant J. 2014 Feb;77(4):511-20. doi: 10.1111/tpj.12398. Epub 2014 Jan 17.
Using floral-dip, tumorigenesis and root callus transformation assays of both germline and somatic cells, we present here results implicating the four major non-homologous and homologous recombination pathways in Agrobacterium-mediated transformation of Arabidopsis thaliana. All four single mutant lines showed similar mild reductions in transformability, but knocking out three of four pathways severely compromised Agrobacterium-mediated transformation. Although integration of T-DNA into the plant genome is severely compromised in the absence of known DNA double-strand break repair pathways, it does still occur, suggesting the existence of other pathways involved in T-DNA integration. Our results highlight the functional redundancy of the four major plant recombination pathways in transformation, and provide an explanation for the lack of strong effects observed in previous studies on the roles of plant recombination functions in transformation.
利用花浸染法、种系细胞和体细胞的肿瘤发生及根愈伤组织转化分析，我们在此展示了与拟南芥农杆菌介导转化中四条主要的非同源和同源重组途径相关的结果。所有四个单突变系在转化能力上均表现出类似的轻度降低，但敲除四条途径中的三条会严重损害农杆菌介导的转化。尽管在没有已知的DNA双链断裂修复途径时，T-DNA整合到植物基因组中会受到严重损害，但它仍然会发生，这表明存在其他参与T-DNA整合的途径。我们的结果突出了四条主要植物重组途径在转化中的功能冗余，并为先前关于植物重组功能在转化中的作用的研究中未观察到强烈效应提供了解释。
