State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.
Institute of Future Agriculture, Northwest A&F University, Yangling, Shaanxi, China.
Mol Plant Pathol. 2024 Sep;25(9):e70006. doi: 10.1111/mpp.70006.
The pathogen Agrobacterium tumefaciens is known for causing crown gall tumours in plants. However, it has also been harnessed as a valuable tool for plant genetic transformation. Apart from the T-DNA, Agrobacterium also delivers at least five virulence proteins into the host plant cells, which are required for an efficient infection. One of these virulence proteins is VirD5. F-box proteins, encoded in the host plant genome or the Ti plasmid, and the ubiquitin/26S proteasome system (UPS) also play an important role in facilitating Agrobacterium infection. Our study identified two Arabidopsis F-box proteins, D5BF1 and D5BF2, that bind VirD5 and facilitate its degradation via the UPS. Additionally, we found that Agrobacterium partially suppresses the expression of D5BF1 and D5BF2. Lastly, stable transformation and tumorigenesis efficiency assays revealed that D5BF1 and D5BF2 negatively regulate the Agrobacterium infection process, showing that the plant F-box proteins and UPS play a role in defending against Agrobacterium infection.
根癌农杆菌是一种已知的植物病原菌，能引起冠瘿瘤。然而，它也被用作植物遗传转化的有价值的工具。除了 T-DNA，根癌农杆菌还将至少五种毒力蛋白递送到宿主植物细胞中，这对于有效的感染是必需的。这些毒力蛋白之一是 VirD5。F-box 蛋白，在宿主植物基因组或 Ti 质粒中编码，以及泛素/26S 蛋白酶体系统（UPS）也在促进根癌农杆菌感染方面发挥重要作用。我们的研究鉴定了两个拟南芥 F-box 蛋白 D5BF1 和 D5BF2，它们与 VirD5 结合，并通过 UPS 促进其降解。此外，我们发现根癌农杆菌部分抑制了 D5BF1 和 D5BF2 的表达。最后，稳定转化和肿瘤发生效率测定表明，D5BF1 和 D5BF2 负调控根癌农杆菌的感染过程，表明植物 F-box 蛋白和 UPS 在抵御根癌农杆菌感染中发挥作用。
