存档标签在鱼类回顾性遗传分析中的应用。

The use of archived tags in retrospective genetic analysis of fish.

作者信息

Bonanomi Sara, Therkildsen Nina Overgaard, Hedeholm Rasmus Berg, Hemmer-Hansen Jakob, Nielsen Einar E

机构信息

Technical University of Denmark, Section for Marine Living Resources, National Institute of Aquatic Resources, Vejlsøvej 39, DK-8600, Silkeborg, Denmark; Greenland Climate Research Centre, Greenland Institute of Natural Resources, Kivioq 2, PO Box 570, 3900, Nuuk, Greenland.

出版信息

Mol Ecol Resour. 2014 May;14(3):616-21. doi: 10.1111/1755-0998.12211. Epub 2013 Dec 30.

DOI:10.1111/1755-0998.12211

PMID:24299474

Abstract

Collections of historical tissue samples from fish (e.g. scales and otoliths) stored in museums and fisheries institutions are precious sources of DNA for conducting retrospective genetic analysis. However, in some cases, only external tags used for documentation of spatial dynamics of fish populations have been preserved. Here, we test the usefulness of fish tags as a source of DNA for genetic analysis. We extract DNA from historical tags from cod collected in Greenlandic waters between 1950 and 1968. We show that the quantity and quality of DNA recovered from tags is comparable to DNA from archived otoliths from the same individuals. Surprisingly, levels of cross-contamination do not seem to be significantly higher in DNA from external (tag) than internal (otolith) sources. Our study therefore demonstrates that historical tags can be a highly valuable source of DNA for retrospective genetic analysis of fish.

摘要

保存在博物馆和渔业机构的鱼类历史组织样本（如鳞片和耳石）的收集物是用于进行回顾性遗传分析的宝贵DNA来源。然而，在某些情况下，仅保存了用于记录鱼类种群空间动态的外部标签。在此，我们测试鱼类标签作为遗传分析DNA来源的实用性。我们从1950年至1968年在格陵兰水域收集的鳕鱼的历史标签中提取DNA。我们表明，从标签中回收的DNA的数量和质量与来自同一批个体的存档耳石中的DNA相当。令人惊讶的是，来自外部（标签）的DNA中的交叉污染水平似乎并不比来自内部（耳石）来源的DNA显著更高。因此，我们的研究表明，历史标签可以成为鱼类回顾性遗传分析的非常有价值的DNA来源。