Wageningen University, Laboratory of Food Chemistry, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands.
Carbohydr Polym. 2014 Jan 30;101:281-8. doi: 10.1016/j.carbpol.2013.09.050. Epub 2013 Sep 19.
Changes of plant cell wall carbohydrate structures occurring during the process to make suitable compost for growth of Agaricus bisporus are unknown. In this paper, composition and carbohydrate structures in compost samples collected during composting and mycelium growth were analyzed. Furthermore, different extracts of compost samples were prepared with water, 1M and 4M alkali and analyzed. At the beginning of composting, 34% and after 16 days of mycelium growth 27% of dry matter was carbohydrates. Carbohydrate composition analysis showed that mainly cellulose and poorly substituted xylan chains with similar amounts and ratios of xylan building blocks were present in all phases studied. Nevertheless, xylan solubility increased 20% over the period of mycelium growth indicating partial degradation of xylan backbone. Apparently, degradation of carbohydrates occurred over the process studied by both bacteria and fungi, mainly having an effect on xylan-chain length and solubility.
在制备适合双孢蘑菇生长的堆肥过程中，植物细胞壁碳水化合物结构的变化尚不清楚。本文分析了堆肥过程中以及菌丝生长过程中收集的堆肥样品的组成和碳水化合物结构，此外还使用水、1M 和 4M 碱制备了不同的堆肥样品提取物并进行了分析。在堆肥开始时，干物质中有 34%是碳水化合物，在 16 天的菌丝生长后，有 27%是碳水化合物。碳水化合物组成分析表明，在所研究的所有阶段中，主要存在纤维素和取代程度较低的木聚糖链，并且木聚糖结构单元的数量和比例相似。然而，在菌丝生长过程中，木聚糖的溶解度增加了 20%，表明木聚糖主链发生了部分降解。显然，在研究过程中，细菌和真菌均导致了碳水化合物的降解，主要影响木聚糖链长和溶解度。
