: 植物多糖和木质素降解的时间动态变化的分泌蛋白组。 （注：原英文表述不太完整规范，翻译出来有些拗口，但尽量忠实原文）
The secretome of : Temporal dynamics of plant polysaccharides and lignin degradation.
作者信息
Duran Katharina, Magnin Joris, America Antoine H P, Peng Mao, Hilgers Roelant, de Vries Ronald P, Baars Johan J P, van Berkel Willem J H, Kuyper Thomas W, Kabel Mirjam A
机构信息
Laboratory of Food Chemistry, Wageningen University & Research, Bornse Weilanden 9, 6708 WG Wageningen, the Netherlands.
Bioscience, Wageningen University & Research, Droevendaalsesteeg 1, 6708 PB Wageningen, the Netherlands.
出版信息
iScience. 2023 Jun 9;26(7):107087. doi: 10.1016/j.isci.2023.107087. eCollection 2023 Jul 21.
Despite substantial lignocellulose conversion during mycelial growth, previous transcriptome and proteome studies have not yet revealed how secretomes from the edible mushroom develop and whether they modify lignin models . To clarify these aspects, secretomes collected throughout a 15-day industrial substrate production and from axenic lab-cultures were subjected to proteomics, and tested on polysaccharides and lignin models. Secretomes (day 6-15) comprised endo-acting and substituent-removing glycoside hydrolases, whereas β-xylosidase and glucosidase activities gradually decreased. Laccases appeared from day 6 onwards. From day 10 onwards, many oxidoreductases were found, with numerous multicopper oxidases (MCO), aryl alcohol oxidases (AAO), glyoxal oxidases (GLOX), a manganese peroxidase (MnP), and unspecific peroxygenases (UPO). Secretomes modified dimeric lignin models, thereby catalyzing syringylglycerol-β-guaiacyl ether (SBG) cleavage, guaiacylglycerol-β-guaiacyl ether (GBG) polymerization, and non-phenolic veratrylglycerol-β-guaiacyl ether (VBG) oxidation. We explored secretomes and insights obtained can help to better understand biomass valorization.
尽管在菌丝体生长过程中木质纤维素有大量转化，但之前的转录组和蛋白质组研究尚未揭示食用菌的分泌蛋白是如何形成的，以及它们是否会修饰木质素模型。为了阐明这些方面，对在15天的工业基质生产过程中收集的以及来自无菌实验室培养物的分泌蛋白进行了蛋白质组学分析，并在多糖和木质素模型上进行了测试。分泌蛋白（第6 - 15天）包括内切作用和去除取代基的糖苷水解酶，而β - 木糖苷酶和葡萄糖苷酶的活性逐渐降低。漆酶从第6天开始出现。从第10天起，发现了许多氧化还原酶，包括众多的多铜氧化酶（MCO）、芳醇氧化酶（AAO）、乙二醛氧化酶（GLOX）、一种锰过氧化物酶（MnP）和非特异性过氧酶（UPO）。分泌蛋白修饰了二聚体木质素模型，从而催化丁香基甘油 - β - 愈创木基醚（SBG）的裂解、愈创木基甘油 - β - 愈创木基醚（GBG）的聚合以及非酚型藜芦基甘油 - β - 愈创木基醚（VBG）的氧化。我们对分泌蛋白进行了探索，所获得的见解有助于更好地理解生物质的增值利用。
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