Analytical R&D, Pfizer BioTherapeutics R&D Pharmaceutical Sciences, 700 Chesterfield Parkway West, Chesterfield, MO 63017, United States.
Analytical R&D, Pfizer BioTherapeutics R&D Pharmaceutical Sciences, 700 Chesterfield Parkway West, Chesterfield, MO 63017, United States.
J Chromatogr A. 2014 Jan 3;1323:97-103. doi: 10.1016/j.chroma.2013.11.010. Epub 2013 Nov 13.
This work describes the use of a reverse phase (RP) column for size-based separation of hydrophilic polysaccharides. The entire separation window (before and after void volume) is used to provide unique separation of polysaccharides from other components in a glycol-conjugate vaccine or complex media of fermentation broth. The technique has also been applied to the separation of polysaccharides of different sizes. The effect of chromatographic parameters including type of packing material (CN, C8 and C18), pore size (80 and 300Å) of stationary phase, concentration of organic solvent on the separation was investigated. In addition, characterization of size-based separation was evaluated by coupling of RP column with multi-angle laser light scattering (MALLs) detector.
本工作描述了反相（RP）柱在基于大小的亲水性多糖分离中的应用。整个分离窗口（死体积前后）用于提供糖缀合物疫苗或发酵液复杂介质中多糖与其他成分的独特分离。该技术还已应用于不同大小多糖的分离。考察了色谱参数对分离的影响，包括填料类型（CN、C8 和 C18）、固定相的孔径（80 和 300Å）、有机溶剂浓度等。此外，通过与多角度激光光散射（MALLs）检测器耦合，对基于大小的分离进行了表征。
