Krawczyk Tomasz, Zalewski Mariusz, Janeta Anna, Hodurek Paweł
1Department of Chemical Organic Technology and Petrochemistry, Faculty of Chemistry, Silesian University of Technology, ul. Krzywoustego 4, 44-100 Gliwice, Poland.
2Wroclaw Medical University, Wybrzeże L. Pasteura 1, 50-367 Wrocław, Poland.
Chromatographia. 2018;81(10):1365-1372. doi: 10.1007/s10337-018-3582-5. Epub 2018 Aug 6.
Meso- and macroporous spherical silica gels of pore sizes in the range of 60-1000 Å and 40-75 µm particle size were investigated as a stationary phase for the separation and purification of polysaccharides and poly(ethylene glycols) (PEGs) of various MWs using an aqueous mobile phase. Sephadex and Bio-Gel were used for comparison as the most common stationary phases for similar purposes. The separation of dextrans of a mean MW = 31 kDa from small molecules (NaCl) was possible with SiO with a pore size of 60-300 Å, but the observed efficiencies of a column of the same size were lower comparing with Sephadex or Bio-Gel. In the case of oxidized alginic acid only SiO of the 60 Å pore size was suitable, while Sephadex, Bio-Gel and other investigated silicas were not efficient. Sephadex and 300-1000 Å SiO offered the possibility of dividing dextrans with MW within the range of 1 MDa-10 kDa into fractions of various MWs, while Bio-Gel and 60 Å SiO were not suitable. The investigated silica gels strongly adsorbed PEGs of MW 2-20 kDa. The amount adsorbed decreased with the increase of pore size and they were not useful as a stationary phase for this class of polymers. An advantage of SiO of the investigated particle size was a very low back pressure comparing with Sephadex. A considerably lower price of silica offers time- and cost-efficient separation of polysaccharides.
研究了孔径在60 - 1000 Å范围内、粒径为40 - 75 µm的中孔和大孔球形硅胶作为固定相，用于使用水性流动相分离和纯化各种分子量的多糖和聚乙二醇（PEG）。使用葡聚糖凝胶（Sephadex）和生物凝胶（Bio - Gel）作为类似用途最常用的固定相进行比较。对于平均分子量为31 kDa的右旋糖酐与小分子（氯化钠）的分离，孔径为60 - 300 Å的硅胶是可行的，但与葡聚糖凝胶或生物凝胶相比，相同尺寸柱的观察效率较低。对于氧化海藻酸，只有孔径为60 Å的硅胶适用，而葡聚糖凝胶、生物凝胶和其他研究的硅胶效率不高。葡聚糖凝胶和孔径为300 - 1000 Å的硅胶能够将分子量在1 MDa - 10 kDa范围内的右旋糖酐分成不同分子量的级分，而生物凝胶和孔径为60 Å的硅胶不适用。所研究的硅胶强烈吸附分子量为2 - 20 kDa的PEG。吸附量随孔径增加而降低，它们不适用于这类聚合物的固定相。所研究粒径的硅胶的一个优点是与葡聚糖凝胶相比背压非常低。硅胶价格低得多，可实现多糖的高效且经济的分离。
