Department of Biology, Faculty of Science, Kanazawa University, Kakuma, 920-11, Kanazawa, Japan.
Photosynth Res. 1995 Nov;46(1-2):301-8. doi: 10.1007/BF00020444.
Several forms (isoproteins) of ferredoxin in roots, leaves, and green and red pericarps in tomato plants (Lycopersicon esculentum Mill.) were earlier identified on the basis of N-terminal amino acid sequence and chromatographic behavior (Green et al. 1991). In the present study, a large scale preparation made possible determination of the full length amino acid sequence of the two ferredoxins from leaves. The ferredoxins characteristic of fruit and root were sequenced from the amino terminus to the 30th residue or beyond. The leaf ferredoxins were confirmed to be expressed in pericarp of both green and red fruit. The ferredoxins characteristic of fruit and root appeared to be restricted to those tissue. The results extend earlier findings in demonstrating that ferredoxin occurs in the major organs of the tomato plant where it appears to function irrespective of photosynthetic competence.
先前基于 N 端氨基酸序列和色谱行为，在番茄植株（Lycopersicon esculentum Mill.）的根、叶以及绿色和红色种皮中鉴定出几种铁氧还蛋白同工型（isoproteins）（Green 等人，1991 年）。在本研究中，大规模制备使得有可能确定来自叶的两种铁氧还蛋白的全长氨基酸序列。从氨基末端到第 30 个残基或更远的位置对果实和根特有的铁氧还蛋白进行测序。确认在绿色和红色果实的种皮中表达叶铁氧还蛋白。果实和根特有的铁氧还蛋白似乎仅限于这些组织。这些结果扩展了早期的发现，表明铁氧还蛋白存在于番茄植物的主要器官中，无论其是否具有光合作用能力，它似乎都在发挥作用。
