Petit F, Glowinski J
Neuropharmacology. 1986 Sep;25(9):1015-21. doi: 10.1016/0028-3908(86)90196-6.
Striatal slices from the rat were continuously superfused with [3H]tyrosine in order to estimate the release of newly synthesized [3H]dopamine [( 3H]DA). Substance P (SP) and neuromedin K (NKB) stimulated the spontaneous release of [3H]DA when used in concentrations (from 10(-9) M to 10(-7) M). The stimulatory effect of substance P (10(-8) M) was prevented completely when slices were superfused with tetrodotoxin (10(-7) M) or the substance P antagonist (D-Arg1,D-Pro2,D-Trp7,9,Leu11) substance P (10(-5 M) indicating that the response evoked by substance P was mediated by substance P receptors but that these were not located on DA nerve terminals. In addition, substance P did not modify the stimulatory effect of acetylcholine (ACh) (10(-5) M) on the spontaneous release of [3H]DA since the effects of substance P (10(-7) M) and ACh (10(-5) M) were additive.
为了评估新合成的[3H]多巴胺（[3H]DA）的释放情况，用[3H]酪氨酸持续灌流大鼠的纹状体切片。当物质P（SP）和神经介素K（NKB）的浓度在10（-9）M至10（-7）M之间时，它们能刺激[3H]DA的自发释放。当切片用河豚毒素（10（-7）M）或物质P拮抗剂（D-Arg1，D-Pro2，D-Trp7，9，Leu11）物质P（10（-5）M）灌流时，物质P（10（-8）M）的刺激作用被完全阻断，这表明物质P引起的反应是由物质P受体介导的，但这些受体并不位于多巴胺神经末梢上。此外，物质P不会改变乙酰胆碱（ACh）（10（-5）M）对[3H]DA自发释放的刺激作用，因为物质P（10（-7）M）和ACh（10（-5）M）的作用是相加的。
