Goad M E, Tryka A F, Witschi H P
Toxicol Lett. 1986 Sep;32(3):173-8. doi: 10.1016/0378-4274(86)90104-9.
Hamsters treated with 0.5 U/kg intratracheal bleomycin and exposed for 24 h to 80% O2 develop acute respiratory failure 72 h after treatment. To examine indirectly the lung epithelial type II cell changes, alterations in pulmonary surfactant was measured. Presence and amount of dipalmitoyl phosphatidyl choline (DDPC) and sphingomyelin (SM) were measured, and the DPPC/SM ratio was determined in brochoalveolar lavage samples from treated and control animals 24, 48, 72, and 96 h after treatment. Hamsters treated with bleomycin and O2 had a significantly decreased DPPC/SM ratio at 72 h, which is the time of onset of respiratory failure. The decreased DPPC/SM ratio may reflect type II cell damage and inhibition of surfactant function by the edema fluid.
经气管内给予0.5 U/kg博来霉素并暴露于80%氧气环境24小时的仓鼠，在治疗后72小时会发生急性呼吸衰竭。为间接检测肺II型上皮细胞的变化，对肺表面活性物质的改变进行了测量。测定了二棕榈酰磷脂酰胆碱（DDPC）和鞘磷脂（SM）的存在及含量，并在治疗后24、48、72和96小时，测定了治疗组和对照组动物支气管肺泡灌洗样本中的DPPC/SM比值。接受博来霉素和氧气治疗的仓鼠在72小时时DPPC/SM比值显著降低，此时正是呼吸衰竭开始的时间。DPPC/SM比值降低可能反映了II型细胞损伤以及水肿液对表面活性物质功能的抑制。
