病毒诱导的番茄基因互补。
Virus-induced gene complementation in tomato.
机构信息
Research Centre for Plant RNA Signaling; College of Life and Environmental Sciences; Hangzhou Normal University; Hangzhou, PR China.
School of Life Sciences; University of Warwick; Coventry, UK.
出版信息
Plant Signal Behav. 2013 Nov;8(11):e27142. doi: 10.4161/psb.27142. Epub 2013 Dec 4.
Abstract
Virus-induced gene complementation (VIGC), a plant virus technology based on Potato virus X for transient overexpression of endogenous genes complemented tomato mutants, resulting in non-ripening fruits to ripen. This efficient "gain-of-function" approach involves no stable transformation, and reveals a fruit-specific transcriptional network that may exist among key transcription factors in modulating tomato ripening. Thus, VIGC represents a novel and feasible strategy for gene functional analysis in plants.
摘要
病毒诱导的基因互补（VIGC）是一种基于马铃薯病毒 X 的植物病毒技术，用于瞬时过表达内源基因来补充番茄突变体，从而使非成熟果实成熟。这种高效的“功能获得”方法不涉及稳定转化，并揭示了一个可能存在于调节番茄成熟的关键转录因子之间的果实特异性转录网络。因此，VIGC 代表了一种在植物中进行基因功能分析的新颖且可行的策略。
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