Institut für Cytologie, Universität Bonn, Ulrich Haberland-Straße 61a, D-5300, Bonn 1, Federal Republik of Germany.
Planta. 1980 Jan;150(2):180-8. doi: 10.1007/BF00582364.
Isolated protoplasmic strands ofPhysarum polycephalum, mounted as a trapeze, show synchronous contraction activities when the isometric tension development of both arms of the trapeze is measured independently of each other. This phase regulation can be experimentally disturbed by temperature changes. Within a permanent gradient, however, the phases become resynchronized. The maximal temperature gradient between both arms allowing a phase resynchronization was approximately 9° C along a distance of 25 mm. The transmission of the signal along the middle piece of the trapeze (which, as the connecting part of both arms, is responsible for signal transmission in phase synchronization) can be influenced by temperature changes. The minimal temperature allowing a signal transmission is 15° C, the maximal temperature approximately 29° C. A morphological investigation of protoplasmic strands mounted as trapezes revealed that the normal architecture of the objects is not influenced by the experimental trapeze arrangement. Permanent thermal gradients induce thermotactic reactions, i.e., a preferred protoplasmic mass transport into one arm of the trapeze. This leads, after several hours, to a morphological asymmetry of the trapeze. In spite of the fact that this reaction limits the temporal use of trapezes within thermal gradients to 2-3 h, the capacity of such strands for phase regulation is not hindered. Thermal gradients are suitable methods for studying the unknown phase-regulating factor and its transmission. As criteria for an intact pathway of signal transmission, the capacity of the trapeze arms to resynchronize as well as to maintain synchronization within a thermal gradient can be used.
当独立测量 trapeze 两翼的等长张力发展时，多形绒泡菌的分离原生质丝显示出同步收缩活动。这种相位调节可以通过温度变化实验性地干扰。然而，在永久梯度内，相位会重新同步。在永久梯度内，相位会重新同步。允许相位重新同步的两个臂之间的最大温度梯度约为 9°C，距离为 25mm。trapeze 中间部分（作为两翼的连接部分，负责相位同步中的信号传输）的信号传输可以受到温度变化的影响。允许信号传输的最小温度为 15°C，最大温度约为 29°C。用 trapeze 安装的原生质丝的形态学研究表明，实验 trapeze 排列不会影响物体的正常结构。永久的温度梯度会引起趋热性反应，即原生质体优先向 trapeze 的一个臂运输。这会导致 trapeze 在几个小时后出现形态不对称。尽管这种反应将 trapeze 在温度梯度内的使用时间限制在 2-3 小时内，但这些丝状物的相位调节能力不会受到阻碍。温度梯度是研究未知相位调节因子及其传输的合适方法。作为信号传输完整途径的标准，可以使用 trapeze 臂重新同步以及在热梯度内保持同步的能力。
