Wohlfarth-Bottermann K E
J Exp Biol. 1979 Aug;81:15-32. doi: 10.1242/jeb.81.1.15.
The plasmodia of Physarum polycephalum show different oscillatory phenomena (time period approximately 1.3 min) in their contraction behaviour and their protoplasmic flow. The force generating system for these phenomena is cytoplasmic actomyosin. The biochemical nature and location(s) of the oscillator(s), i.e. the clock governing these phenomena are unknown. The following locations are discussed as possible sites of the oscillator: (1) cytoplasmic actomyosin, (2) the energy supply system, (3) inner Ca2+ stores, and (4) the plasmalemma, which must be involved at least in modulating the force generated by the contractile machinery during a chemotactic response. The following oscillatory phenomena were used to assess the effects of externally and internally applied substances (e.g. calcium antagonistic drugs, caffeine, D2O) on oscillating force output: (1) persistance of longitudinal contractile activity of veins (for external application of test substances), (2) persistance of radial activity of veins (for internal application of the test substances), (3) de novo generation of contractile activity in protoplasmic drops (external application). The data seem to exclude rhythmical Ca2+, Na+ or K+ transport across the plasmalemma as a triggering function for the oscillation. Contractile activity seems to represent a spontaneous, endogeneous oscillation which can be modulated via the plasmalemma during chemotaxis.
多头绒泡菌的原质团在其收缩行为和原生质流动方面表现出不同的振荡现象（周期约为1.3分钟）。这些现象的力产生系统是细胞质肌动球蛋白。振荡器（即控制这些现象的时钟）的生化性质和位置尚不清楚。以下位置被讨论为振荡器的可能位点：（1）细胞质肌动球蛋白，（2）能量供应系统，（3）细胞内钙库，以及（4）质膜，其至少必须参与在趋化反应期间调节收缩机制产生的力。以下振荡现象被用于评估外部和内部施加的物质（例如钙拮抗剂、咖啡因、重水）对振荡力输出的影响：（1）静脉纵向收缩活动的持续性（用于测试物质的外部施加），（2）静脉径向活动的持续性（用于测试物质的内部施加），（3）原生质滴中收缩活动的重新产生（外部施加）。数据似乎排除了跨质膜的节律性钙、钠或钾运输作为振荡的触发功能。收缩活动似乎代表一种自发的、内源性振荡，在趋化过程中可通过质膜进行调节。
