Department of Physiology and Environmental Science, University of Nottingham Faculty of Agricultural Science, Sutton Bonington, LE12 5RD, Loughborough, U.K..
Plant Mol Biol. 1985 May;5(3):137-47. doi: 10.1007/BF00015677.
Gene expression during the ripening of tomato fruit was investigated by cDNA cloning and hybrid-select translation. A cDNA library was prepared from poly(A)-containing mRNA from ripe tomato fruit and sreened by differential hybridization. 146 ripening-related cDNA clones were found. Eleven groups and eight unique clones have been identified so far. The sizes of the cloned cDNA inserts were determined and type-members for seven groups were used in hybrid selection experiments. Six of the seven clones encode translation products corresponding to six ripening related polypeptides detected previously by in vitro translation of total cytoplasmic RNA (14). One cDNA group codes for a Mr 48 000 protein that was identified as polygalacturonase on the basis of immunoprecipitation with specific antiserum raised against tomato polygalacturonase. re]19840918 rv]19850613 ac]19850618.
采用 cDNA 克隆和杂交选择翻译的方法研究了番茄果实成熟过程中的基因表达。从富含 poly(A)的成熟番茄果实 mRNA 中制备 cDNA 文库，并通过差异杂交进行筛选。发现了 146 个与成熟相关的 cDNA 克隆。迄今为止，已经鉴定出 11 个组和 8 个独特的克隆。确定了克隆 cDNA 插入物的大小，并在杂交选择实验中使用了七个组的型成员。七个克隆中的六个编码与以前通过总细胞质 RNA 的体外翻译检测到的六个与成熟相关的多肽相对应的翻译产物(14)。一个 cDNA 组编码 Mr 48000 蛋白，该蛋白基于针对番茄多聚半乳糖醛酸酶的特异性抗血清的免疫沉淀被鉴定为多聚半乳糖醛酸酶。re]19840918 rv]19850613 ac]19850618.
