Grierson D, Tucker G A, Keen J, Ray J, Bird C R, Schuch W
Nucleic Acids Res. 1986 Nov 11;14(21):8595-603. doi: 10.1093/nar/14.21.8595.
The 2a isoenzyme of tomato polygalacturonase was purified from ripe fruit and characterised. The N-terminal amino acid sequence of the protein was determined in order to identify polygalacturonase cDNA clones. The nucleotide sequence of a ripening-related cDNA (pTOM 6) was determined and found to encode the N-terminal sequence of mature polygalacturonase 2a. The complete open reading frame encodes a polypeptide of molecular weight 50,051, including a putative pre-sequence of 71 amino acids.
番茄多聚半乳糖醛酸酶的2a同工酶从成熟果实中纯化并进行了表征。测定了该蛋白质的N端氨基酸序列，以鉴定多聚半乳糖醛酸酶cDNA克隆。测定了一个与成熟相关的cDNA（pTOM 6）的核苷酸序列，发现其编码成熟多聚半乳糖醛酸酶2a的N端序列。完整的开放阅读框编码一个分子量为50,051的多肽，包括一个由71个氨基酸组成的假定前导序列。
