Friedrich Miescher-Institut, Basel, Switzerland.
Theor Appl Genet. 1979 Nov;55(6):257-62. doi: 10.1007/BF00265362.
A method was devised for the biochemical selection of immature, haploid Zea mays embryos using Adh1 (-) and either the Stock 6 or indeterminate gametophyte (ig in W23) high haploid-inducing systems. Haploid (Adh1 (-)) embryos survived exposure to levels of allyl alcohol which killed diploid (Adh1 (+)/Adh1 (-)) embryos. Of the total surviving embryos which were examined cytologically 15% (using ig) and 22% (using Stock 6) were haploid. In two experiments with Stock 6, 100% of the surviving embryos were haploid. To obtain maximum effectiveness of Stock 6 and ig, Adh1 (-) was transferred to stock 6 and W23 backgrounds. Immature, haploid embryos are being used to develop haploid, morphogenic tissue cultures of Zea mays.
设计了一种使用 Adh1(-)和 Adh1(-)Stock 6 或不定配子体（W23 中的 ig）高单倍体诱导系统对未成熟的单倍体玉米胚胎进行生化选择的方法。单倍体（Adh1(-)）胚胎在暴露于丙烯醇水平下存活，而该水平会杀死二倍体（Adh1(+)/Adh1(-)）胚胎。在使用 ig 的总存活胚胎中，有 15%的胚胎经细胞学检查为单倍体，而在使用 Stock 6 的情况下则有 22%的胚胎为单倍体。在两项使用 Stock 6 的实验中，100%的存活胚胎为单倍体。为了使 Stock 6 和 ig 的效果最大化，将 Adh1(-)转移到 Stock 6 和 W23 背景下。正在使用未成熟的单倍体胚胎来开发玉米的单倍体、形态发生组织培养物。
