Rousselin P, Lepingle A, Faure J D, Bitoun R, Caboche M
Laboratoire de Biologie Cellulaire, INRA Centre de Versailles, France.
Mol Gen Genet. 1990 Jul;222(2-3):409-15. doi: 10.1007/BF00633847.
Six independent mutant lines of Nicotiana plumbaginifolia resistant to ethanol, designated E3, E8, E101, E112, E144 and E251, were isolated as germinating seedlings on selective medium. In all cases, resistance to ethanol was conferred by a single recessive nuclear mutation at the same locus. Mutant seeds and pollen lacked detectable ADH activity, with the exception of E251 where a residual activity was detected. An antiserum directed against Arabidopsis thaliana ADH detected an ADH-related polypeptide of 44 kDa present in wild-type seeds and, to a lesser extent, in the seeds of the leaky mutant E251. No ADH-related polypeptide could be detected in seeds of the other mutants. However, all of them had a nearly normal level of ADH mRNA except one which did not synthesize any mRNA. These results suggest that these ethanol-resistant mutants are impaired in one of the structural genes coding for alcohol dehydrogenase. The corresponding locus has been designated Adh1.
从发芽的幼苗中在选择培养基上分离出六个独立的对乙醇具有抗性的烟草突变株系，分别命名为E3、E8、E101、E112、E144和E251。在所有情况下，对乙醇的抗性由同一基因座上的单个隐性核突变赋予。除了检测到有残余活性的E251外，突变种子和花粉均未检测到可检测的乙醇脱氢酶（ADH）活性。一种针对拟南芥ADH的抗血清检测到在野生型种子中存在一种44 kDa的ADH相关多肽，在渗漏突变体E251的种子中也有较低程度的存在。在其他突变体的种子中未检测到ADH相关多肽。然而，除了一个不合成任何mRNA的突变体外，它们所有的ADH mRNA水平几乎正常。这些结果表明，这些抗乙醇突变体在编码乙醇脱氢酶的结构基因之一中存在缺陷。相应的基因座已被命名为Adh1。
