Institut de Biologie Physico-chimique, 75005, Paris, France.
Plant Mol Biol. 1986 May;6(3):151-60. doi: 10.1007/BF00021484.
We have examined 78 chloroplast mutants of Chlamydomonas reinhardii lacking photosystem II activity. Most of them are unable to synthesize the 32 Kdalton protein. Analysis of 22 of these mutants reveals that they have deleted both copies of the psbA gene (which codes for the 32 Kdalton protein) in their chloroplast genome. Although these mutants are able to synthesize and to integrate the other photosystem II polypeptides in the thylakoid membranes, they are unable to assemble a stable functional photosystem II complex. The 32 Kprotein appears therefore to play an important role not only in photosystem II function, but also in stabilizing this complex.
我们研究了 78 株缺乏光系统 II 活性的莱茵衣藻叶绿体突变体。它们大多数都不能合成 32kDa 蛋白。对其中 22 株突变体的分析表明，它们的叶绿体基因组中 psbA 基因（编码 32kDa 蛋白）的两个拷贝都缺失了。尽管这些突变体能够在类囊体膜中合成和整合其他光系统 II 多肽，但它们不能组装一个稳定的、有功能的光系统 II 复合物。因此，32kDa 蛋白不仅在光系统 II 的功能中起着重要作用，而且在稳定这个复合物中也起着重要作用。
