两种植物病毒外壳蛋白信使克隆 DNA 的生物活性转录物。

Biologically active transcripts of cloned DNA of the coat protein messenger of two plant viruses.

机构信息

Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands.

出版信息

Plant Mol Biol. 1986 Jul;6(4):281-8. doi: 10.1007/BF00015234.

Abstract

To initiate infection, a mixture of the three genomic RNAs of alfalfa mosaic virus (AIMV) has to be supplemented with a small amount of coat protein or RNA 4, the subgenomic messenger for coat protein. The possibility to replace RNA 4 in the inoculum by in vitro synthesized transcripts of a cloned DNA copy of the coat protein cistron was investigated using the SP6 transcription system. Transcripts with or without the cap structure m(7)G(5')ppp(5')G were both translated in vitro in viral coat protein, but only capped transcripts yielded an infectious mixture when added to the AIMV genomic RNAs. This indicates that the cap structure is essential to the in vivo translatin of RNA 4. Similar results were obtained with RNAs transcribed in vitro from a DNA copy of the putative coat protein cistron of tobacco streak virus (TSV). re]19850822 rv]19851203 ac]19860114.

摘要

为了引发感染，必须在紫花苜蓿花叶病毒（AIMV）的三个基因组 RNA 混合物中补充少量外壳蛋白或 RNA4，即外壳蛋白的亚基因组信使。使用 SP6 转录系统研究了用克隆的外壳蛋白顺反子的 DNA 拷贝的体外合成转录本替代接种物中 RNA4 的可能性。具有或不具有帽结构 m(7)G(5')ppp(5')G 的转录本均在体外翻译为病毒外壳蛋白，但只有加帽转录本在添加到 AIMV 基因组 RNA 时产生感染性混合物。这表明帽结构对于 RNA4 的体内翻译是必需的。用来自烟草条纹病毒（TSV）的外壳蛋白顺反子的 DNA 拷贝的体外转录的 RNA 获得了类似的结果。re]19850822 rv]19851203 ac]19860114。