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通过凝胶电泳和银染增强法对鸡组织锌-金属硫蛋白进行定量分析。

Quantitation of chick tissue zinc-metallothionein by gel electrophoresis and silver stain enhancement.

作者信息

Lin L Y, McCormick C C

出版信息

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1986;85(1):75-84. doi: 10.1016/0742-8413(86)90054-x.

DOI:10.1016/0742-8413(86)90054-x
PMID:2430754
Abstract

Discontinuous gradient gel electrophoretic systems were developed to quantitate zinc-metallothionein (Zn-MT) in chick tissues, liver and pancreas. Gels were stained with Coomassie Blue initially, then enhanced by silver stain. At least 4 micrograms of Zn-MT could be detected after Coomassie Blue stain, and 1 microgram Zn-MT detected following silver stain enhancement. Significant linearity (correlation coefficient = 0.99) of a standard curve was established in the Coomassie Blue stained gels. The results of our experiment suggest that electrophoretic analysis is a simple and feasible method for the quantitation and identification of Zn-MT in chick tissues.

摘要

开发了不连续梯度凝胶电泳系统来定量检测鸡组织（肝脏和胰腺）中的锌金属硫蛋白（Zn-MT）。凝胶最初用考马斯亮蓝染色，然后用银染增强。考马斯亮蓝染色后至少可检测到4微克的Zn-MT，银染增强后可检测到1微克的Zn-MT。在考马斯亮蓝染色的凝胶中建立了标准曲线的显著线性关系（相关系数=0.99）。我们的实验结果表明，电泳分析是一种简单可行的定量和鉴定鸡组织中Zn-MT的方法。

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