Mello-Filho A C, Chubatsu L S, Meneghini R
Department of Biochemistry, University of São Paulo, Brazil.
Biochem J. 1988 Dec 1;256(2):475-9. doi: 10.1042/bj2560475.
Chinese hamster cells (V79) resistant to high concentrations of Cd2+ in the medium were obtained by using the procedure of Beach & Palmiter [(1981) Proc. Natl. Acad. Sci. U.S.A. 78, 2110-2114], which in mouse led to amplification of metallothionein (MT) genes and to an enrichment in cellular MT. The Cd-resistant V79 clones isolated were significantly more resistant than parental cells to oxidative stress by extracellular H2O2 or a mixture of H2O2 and superoxide anion (O2-) generated by xanthine oxidase plus acetaldehyde. On a per-cell basis, there was no difference between the two cells in their total H2O2-decomposing or O2-(-)dismutating activity. The most likely explanation is that an enrichment in MT content in the Cd-resistant cells was responsible for this effect, because of the antioxidant properties already described for this protein.
通过采用比奇和帕尔米特[（1981年）《美国国家科学院院刊》78卷，2110 - 2114页]的方法，获得了对培养基中高浓度Cd2 +具有抗性的中国仓鼠细胞（V79）。该方法在小鼠中会导致金属硫蛋白（MT）基因扩增以及细胞内MT富集。分离得到的抗镉V79克隆比亲代细胞对细胞外H2O2或黄嘌呤氧化酶加乙醛产生的H2O2和超氧阴离子（O2-）混合物引起的氧化应激具有显著更强的抗性。以每个细胞为基础，两种细胞在其总的H2O2分解或O2-歧化活性方面没有差异。最可能的解释是，抗镉细胞中MT含量的富集导致了这种效应，因为该蛋白已被描述具有抗氧化特性。
