Sun G G, Zhang J, Hu W N
Department of Chemoradiotherapy, Tangshan People's Hospital, No. 65, Shengli Road, Lunan District, Tangshan, 063000, Hebei Province, China.
Tumour Biol. 2014 Apr;35(4):3339-46. doi: 10.1007/s13277-013-1440-5. Epub 2013 Dec 3.
This study aimed to analyze the expression, clinical significance of cyclin G2 (CCNG2) in colorectal carcinoma, and the biological effect in its cell line by CCNG2 overexpression. Immunohistochemistry and Western blot were used to analyze CCNG2 protein expression in colorectal cancer and to study the influence of the upregulated expression of CCNG2 that might be found on SW480 cell biological effect. We found that the level of CCNG2 protein expression was significantly lower in colorectal cancer tissue than normal tissues (P < 0.05). The level of CCNG2 was correlated with T stages, lymph node metastasis, clinic stage, and histological grade (P < 0.05). Loss of CCNG2 expression correlated significantly with poor overall survival time by Kaplan-Meier analysis (P < 0.05). The result of biological function has shown that SW480 cell-transfected CCNG2 had a lower survival fraction, higher percentage of the G0/G1 phases, and lower CDK2 protein expression compared with SW480 cell-untransfected CCNG2 (P < 0.05).
本研究旨在分析细胞周期蛋白G2（CCNG2）在结直肠癌中的表达、临床意义，以及通过CCNG2过表达对其细胞系的生物学效应。采用免疫组织化学和蛋白质印迹法分析CCNG2蛋白在结直肠癌中的表达，并研究可能上调的CCNG2表达对SW480细胞生物学效应的影响。我们发现，结直肠癌组织中CCNG2蛋白表达水平显著低于正常组织（P<0.05）。CCNG2水平与T分期、淋巴结转移、临床分期和组织学分级相关（P<0.05）。通过Kaplan-Meier分析，CCNG2表达缺失与总体生存时间差显著相关（P<0.05）。生物学功能结果显示，与未转染CCNG2的SW480细胞相比，转染CCNG2的SW480细胞存活分数更低，G0/G1期百分比更高，CDK2蛋白表达更低（P<0.05）。
