Massi G, Coli A, Marano G
Eur J Obstet Gynecol Reprod Biol. 1986 Oct;23(1-2):13-23. doi: 10.1016/0028-2243(86)90100-0.
The presence of various proteins (mostly serum proteins) has been investigated in the chorionic villi of human placentas in the first term of gestation. The peroxidase-antiperoxidase method was employed. In normal chorionic tissue, i.e. obtained from therapeutic abortions, a positive staining for alpha 1-antitrypsin (A1AT), alpha 1-antichymotrypsin (A1AC), albumin and IgG was observed in syncytiotrophoblast but not in cytotrophoblast. Staining for other proteins, including fibrinogen, antithrombin III (AT III), lysozyme, ferritin, orosomucoid, carcinoembryonic antigen (CEA), alpha-fetoprotein (AFP), IgA, IgM and alpha 2-macroglobulin (A2M), was always negative in the trophoblast. Similar results were obtained in only a few cases of tissue obtained from spontaneous abortions which occurred during the first term of pregnancy. In the majority of spontaneous abortions a different immunohistochemical pattern was observed. The syncytiotrophoblast was immunonegative in the majority of cases, especially for albumin, whereas the cytotrophoblast showed a positive (although variable) reaction to A1AT, A1AC, albumin, IgG and orosomucoid antibodies. There is no evidence to indicate whether these differences are the cause or the secondary result of the spontaneous abortions, but we can hypothesize that they reflect an alteration of pinocytic functions of the trophoblast during the spontaneous abortions.
在妊娠早期，对人胎盘绒毛膜中的各种蛋白质（主要是血清蛋白）进行了研究。采用过氧化物酶-抗过氧化物酶法。在正常绒毛膜组织中，即从治疗性流产获得的组织中，在合体滋养层细胞中观察到α1-抗胰蛋白酶（A1AT）、α1-抗糜蛋白酶（A1AC）、白蛋白和IgG呈阳性染色，而在细胞滋养层细胞中未观察到。对包括纤维蛋白原、抗凝血酶III（AT III）、溶菌酶、铁蛋白、类粘蛋白、癌胚抗原（CEA）、甲胎蛋白（AFP）、IgA、IgM和α2-巨球蛋白（A2M）在内的其他蛋白质的染色，在滋养层细胞中始终为阴性。在妊娠早期发生的少数自然流产病例中也得到了类似结果。在大多数自然流产病例中，观察到不同的免疫组织化学模式。在大多数病例中，合体滋养层细胞呈免疫阴性，尤其是白蛋白，而细胞滋养层细胞对A1AT、A1AC、白蛋白、IgG和类粘蛋白抗体呈阳性（尽管程度不同）反应。没有证据表明这些差异是自然流产的原因还是继发结果，但我们可以推测，它们反映了自然流产期间滋养层细胞胞饮功能的改变。
