Mallett A K, Bearne C A, Rowland I R
Food Chem Toxicol. 1986 Jun-Jul;24(6-7):743-7. doi: 10.1016/0278-6915(86)90174-2.
To investigate the involvement of bacterial enzyme activities in the biotransformation of xenobiotic compounds, we have developed a simulation of the rat hindgut microflora in vitro. This mixed bacterial population exhibits many similarities to the native rat flora, and the diversity of bacterial species and the activity of a number of hydrolytic and reductive enzymes (e.g. azoreductase, beta-glucosidase, beta-glucuronidase, nitrate reductase and nitroreductase) are reproduced in the culture at levels similar to those found in the large intestine. The flora have been found to respond to an anutrient (cyclamate) or to host products (bile acids) with changes in enzyme activity, and to metabolize the azo dye Brown HT to metabolites qualitatively similar to those found in the faeces after oral administration to the rat. The experiments demonstrate that the bacterial population of the large intestine of the rat may be successfully cultured in vitro and provides and alternative to animal studies for the investigation of foreign compound metabolism by the flora.
为了研究细菌酶活性在异源生物化合物生物转化中的作用，我们开发了一种体外模拟大鼠后肠微生物群的方法。这种混合细菌群体与天然大鼠菌群有许多相似之处，并且细菌种类的多样性以及多种水解酶和还原酶（如偶氮还原酶、β-葡萄糖苷酶、β-葡萄糖醛酸酶、硝酸还原酶和硝基还原酶）的活性在培养物中的再现水平与在大肠中发现的水平相似。已发现该菌群会因营养物质（甜蜜素）或宿主产物（胆汁酸）而发生酶活性变化，并将偶氮染料布朗HT代谢为与大鼠口服给药后粪便中发现的代谢物在质量上相似的代谢物。实验表明，大鼠大肠中的细菌群体可以在体外成功培养，为通过菌群研究外来化合物代谢提供了一种替代动物研究的方法。
