Department of Oral and Maxillofacial Surgery, Nara Medical University (YM, TK).
J Histochem Cytochem. 2014 Mar;62(3):211-26. doi: 10.1369/0022155413517700. Epub 2013 Dec 5.
The expression of glucocorticoid receptors (GRs) was investigated immunohistochemically in two different lineages of oligodendrocytes, using carbonic anhydrase (CA) II and neuron glial antigen (NG) 2 as markers of mature oligodendrocytes and oligodendrocyte progenitors, respectively. We focused on the gray matter regions, including CA1, CA3 and the dentate gyrus of the hippocampus, the primary somatosensory cortex barrel field and the basolateral amygdala, and the white matter regions, including the corpus callosum, external capsule and fimbria of the hippocampus. More than 80% of CAII-immunoreactive (IR) cells and more than 95% of NG2-IR cells expressed GRs in various regions of the brain. In contrast, neither CAII-IR cells nor NG2-IR cells expressed mineralocorticoid receptors (MRs) in the same regions. The intensity of GR expression was drastically reduced in CA II-IR cells and NG2-IR cells in the same regions in adrenalectomized mice. Finally, steroid receptor co-activator (SRC)-1 and p300, both of which are cofactors for GR, were expressed in the gray and white matter regions in NG2-IR cells, but not in CAII-IR cells. These results suggest that the expression of GRs in oligodendrocytes and their progenitor cells mediates several functions in vivo, including differentiation and myelination, as a major target of glucocorticoids and their cofactors.
使用碳酸酐酶（CA） II 和神经元胶质抗原（NG） 2 作为成熟少突胶质细胞和少突胶质前体细胞的标志物，我们用免疫组织化学方法研究了糖皮质激素受体（GR）在两种不同少突胶质细胞谱系中的表达。我们重点关注灰质区域，包括海马 CA1、CA3 和齿状回、初级体感皮层桶状野和基底外侧杏仁核，以及白质区域，包括胼胝体、外囊和海马伞。在大脑的不同区域，超过 80%的 CAII-免疫反应（IR）细胞和超过 95%的 NG2-IR 细胞表达 GR。相比之下，在同一区域，CAII-IR 细胞和 NG2-IR 细胞均不表达盐皮质激素受体（MR）。在肾上腺切除术小鼠的相同区域，CA II-IR 细胞和 NG2-IR 细胞中 GR 表达的强度明显降低。最后，类固醇受体共激活因子（SRC）-1 和 p300 在 NG2-IR 细胞的灰质和白质区域表达，但在 CAII-IR 细胞中不表达。这些结果表明，GR 在少突胶质细胞及其前体细胞中的表达介导了体内的几种功能，包括分化和髓鞘形成，作为糖皮质激素及其共因子的主要靶标。
