Department of Botany, Plant Science Laboratories, University of Reading, Whiteknights, RG6 2AS, Reading, UK.
Planta. 1980 Mar;148(2):138-45. doi: 10.1007/BF00386414.
The structural events accompanying the maturation of the pistil of Petunia hybrida have been studied in detail, together with the changes in the protein spectrum of the transmitting tissue that occur over this period. These events have been considered in terms of the acquisition of the self-incompatibility response, which occurs while the pistil is enclosed in the bud. Apart from several minor differences, the young pistils differ only from the mature in that their transmitting tissue cells fail to respond to pollination by undergoing characteristic ultrastructural changes. Data from electrofocusing indicates that several proteins, mobilised in the mature transmitting tissue some three hours after pollination, are absent from bud pistils. It is proposed that the pollination-stimulated release of certain polypeptides, believed to be involved in the self-incompatibility response, does not take place in young pistils. These observations are considered with reference to currently-accepted models of the operation of the self-incompatibility mechanism in Petunia.
已详细研究了矮牵牛雌蕊成熟伴随的结构事件，以及在此期间传递组织蛋白谱的变化。这些事件是根据雌蕊在芽中封闭时发生的自交不亲和反应的获得来考虑的。除了几个小的差异外，年轻的雌蕊与成熟的雌蕊不同之处仅在于它们的传递组织细胞未能通过经历特征性的超微结构变化来响应授粉。来自聚焦电泳的数据表明，授粉后约 3 小时在成熟的传递组织中动员的几种蛋白质不存在于芽雌蕊中。据推测，在幼雌蕊中，某些被认为参与自交不亲和反应的多肽的授粉刺激释放不会发生。这些观察结果与目前公认的矮牵牛自交不亲和机制作用模型有关。
