Herrero M, Dickinson H G
J Cell Sci. 1981 Feb;47:365-83. doi: 10.1242/jcs.47.1.365.
Pollen tubes formed following compatible and incompatible intraspecific matings in Petunia have been examined with light and electron microscopes. Compatible and incompatible tubes develop in an identical fashion on the stigma but, on entry into the top 1 mm of the stylar transmitting tissue changes occur both to the cytology of the tubes and their rates of growth. The early cytological changes are common to tubes of both compatibilities but, although both types of tube accelerate on entry into the style, incompatible tubes grow more slowly than compatible. Cytological differences became apparent between compatible and incompatible tubes following a short period of growth in the style, the latter possessing thicker cell walls and a cytoplasm packed with both organelles and reserves. Incompatible tubes subsequently burst or simply cease growth and die. The characteristic image afforded by this cytoplasm resembles that or burst or dead compatible tubes, except in that proportions of the cell components may differ. These data are discussed in terms of current models proposed to explain pollen tube growth and the operation of the self-incompatibility response in Petunia.
利用光学显微镜和电子显微镜对矮牵牛种内亲和与不亲和交配后形成的花粉管进行了研究。亲和与不亲和的花粉管在柱头上以相同的方式生长，但进入花柱顶部1毫米的传粉组织后，花粉管的细胞学特征及其生长速率都会发生变化。早期的细胞学变化在两种亲和性的花粉管中都很常见，尽管两种类型的花粉管进入花柱后都会加速生长，但不亲和的花粉管比亲和的花粉管生长得更慢。在花柱中生长一段时间后，亲和与不亲和的花粉管之间出现了明显的细胞学差异，后者具有更厚的细胞壁，细胞质中充满了细胞器和贮藏物。不亲和的花粉管随后破裂或干脆停止生长并死亡。这种细胞质呈现出的特征图像类似于破裂或死亡的亲和花粉管，只是细胞成分的比例可能有所不同。根据目前提出的解释矮牵牛花粉管生长和自交不亲和反应机制的模型对这些数据进行了讨论。
