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绿硫菌 Chlorella tepidum 中膜结合态和可溶细胞色素 c 的光氧化。

Photo-oxidation of membrane-bound and soluble cytochromec in the green sulfur bacteriumChlorobium tepidum.

机构信息

Department of Biology, Tokyo Metropolitan University, Minamiohsawa 1-1, Hachioji, 192-03, Tokyo, Japan.

出版信息

Photosynth Res. 1994 Jul;41(1):125-34. doi: 10.1007/BF02184152.

DOI:10.1007/BF02184152
PMID:24310018
Abstract

We studied the photosynthetic electron transfer system of membrane-bound and soluble cytochromec inChlorobium tepidum, a thermophilic green sulfur bacterium, using whole cells and membrane preparations. Sulfide and thiosulfate, physiological electron donors, enhanced flash-induced photo-oxidation ofc-type cytochromes in whole cells. In membranes,c-553 cytochromes with two (or three) heme groups served as immediate electron donors for photo-oxidized bacteriochlorophyll (P840) in the reaction center, and appeared to be closely associated with the reaction center complex. The membrane-bound cytochromec-553 had anE m-value of 180 mV. When isolated soluble cytochromec-553, which has an apparent molecular weight of 10 kDa and seems to correspond to the cytochromec-555 inChlorobium limicola andChlorobium vibrioforme, was added to a membrane suspension, rapid photo-oxidation of both soluble and membrane-bound cytochromesc-553 was observed. The oxidation of soluble cytochromec-553 was inhibited by high salt concentrations. In whole cells, photo-oxidation was observed in the absence of exogenous electron donors and re-reduction was inhibited by stigmatellin, an inhibitor of the cytochromebc complex. These results suggest that the role of membrane-bound and soluble cytochromec inC. tepidum is similar to the role of cytochromec in the photosynthetic electron transfer system of purple bacteria.

摘要

我们研究了嗜热绿硫菌（Chlorobium tepidum）膜结合和可溶性细胞色素 c 的光合电子传递系统，使用了全细胞和膜制剂。硫化物和硫代硫酸盐作为生理电子供体，增强了全细胞中 c 型细胞色素的闪光诱导光氧化。在膜中，具有两个（或三个）血红素基团的 c-553 细胞色素作为反应中心中光氧化菌叶绿素（P840）的直接电子供体，并似乎与反应中心复合物密切相关。膜结合的细胞色素 c-553 的 E m 值为 180 mV。当分离出的可溶性细胞色素 c-553（分子量约为 10 kDa，似乎与 Chlorobium limicola 和 Chlorobium vibrioforme 中的细胞色素 c-555 相对应）添加到膜悬浮液中时，观察到可溶性和膜结合的细胞色素 c-553 的快速光氧化。高盐浓度抑制可溶性细胞色素 c-553 的氧化。在全细胞中，在没有外源电子供体的情况下观察到光氧化，并且抑制了细胞色素 bc 复合物抑制剂 stigmatellin 的再还原。这些结果表明，膜结合和可溶性细胞色素 c 在 C. tepidum 中的作用类似于紫细菌光合作用电子传递系统中细胞色素 c 的作用。

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