Dracheva S, Williams J C, Van Driessche G, Van Beeumen J J, Blankenship R E
Department of Chemistry and Biochemistry, Arizona State University, Tempe 85287-1604.
Biochemistry. 1991 Dec 3;30(48):11451-8. doi: 10.1021/bi00112a012.
The complete nucleotide sequence of the cytochrome c-554 gene from the green photosynthetic bacterium Chloroflexus aurantiacus has been determined. The derived amino acid sequence showed that the cytochrome precursor protein consists of 414 residues and contains 4-Cys-X-X-Cys-His- heme binding motifs. The only regions of the cytochrome c-554 sequence that were found to be significantly similar to the sequences of cytochromes from other organisms were the heme binding sites. The highest similarity was found with the heme binding segments in the four-heme reaction center cytochrome subunit from the purple photosynthetic bacterium Rhodopseudomonas viridis. The importance of this similarity for the evolutionary relationship between Chloroflexus and the purple bacteria is discussed.
已测定绿色光合细菌橙黄嗜热栖热菌细胞色素c-554基因的完整核苷酸序列。推导的氨基酸序列表明，细胞色素前体蛋白由414个残基组成，包含4个半胱氨酸-X-X-半胱氨酸-组氨酸-血红素结合基序。细胞色素c-554序列中唯一被发现与其他生物体细胞色素序列有显著相似性的区域是血红素结合位点。与紫色光合细菌绿脓杆菌四血红素反应中心细胞色素亚基中的血红素结合片段相似度最高。讨论了这种相似性对嗜热栖热菌和紫色细菌进化关系的重要性。
