Department of Neurosurgery, Ulsan University College of Medicine Asan Medical Center, Seoul, Korea.
Medical Research Institute, Chung-Ang University College of Medicine, Seoul, Korea.
Cancer Gene Ther. 2013 Dec;20(12):678-82. doi: 10.1038/cgt.2013.69. Epub 2013 Dec 6.
Neural stem cells (NSCs) led to the development of a novel strategy for delivering therapeutic genes to brain tumors. Human NSCs expressing rabbit carboxyl esterase (F3.CE), which activates CPT-11, significantly inhibit the growth of A549 human non-small cell lung adenocarcinoma cells in the presence of CPT-11 in vitro and in vivo. F3.CE cells migrated selectively into the brain metastases located in the opposite hemisphere. The treatment also significantly decreased tumor volume in immune-deficient mice bearing lung cancer when F3.CE cells were transplanted into the contralateral hemisphere. The survival of tumor-bearing animals was significantly prolonged by the treatment with F3.CE and CPT-11. This strategy could be considered as an effective treatment regimen for lung cancer brain metastases.
神经干细胞（NSCs）为向脑肿瘤递送治疗基因带来了一种新的策略。表达兔羧酸酯酶（F3.CE）的人 NSCs，可激活 CPT-11，在体外和体内均能显著抑制 A549 人非小细胞肺癌腺癌细胞的生长。F3.CE 细胞选择性迁移到位于对侧半球的脑转移部位。当 F3.CE 细胞被移植到对侧半球时，该治疗方法还显著减少了免疫缺陷小鼠肺癌模型中的肿瘤体积。F3.CE 和 CPT-11 的联合治疗显著延长了荷瘤动物的生存期。该策略可被视为治疗肺癌脑转移的有效治疗方案。
