Skaleric U, Zajsek P, Cvetko E, Lah T, Babnik J
J Clin Periodontol. 1986 Oct;13(9):833-6. doi: 10.1111/j.1600-051x.1986.tb02238.x.
The concentration of total, free and bound (complexed forms) of alpha 2-macroglobulin was measured by crossimmunoelectrophoresis in patients with different degrees of periodontal disease as indicated by the gingival index (GI) and the proportion of alveolar bone loss (ABL). The concentration of total alpha 2-M (bound and free forms) is lower in gingival fluid taken from sites with more inflamed gingivae. Its concentration decreases with an increase in the pocket depth and the alveolar bone loss at the sites of fluid collection. The concentration of alpha 2-M bound form, i.e., the presence of alpha 2-M: protease complexes, in gingival fluid is low or absent at the sites with more pronounced bone loss (above 15%). Our results support the hypothesis that unbalanced protease activity damages the periodontal tissue. Not only the proteolytic but also inhibitory activities are altered and correspond to the severity of the disease.
通过交叉免疫电泳法测定了不同程度牙周疾病患者中α2-巨球蛋白的总量、游离形式和结合（复合形式）的浓度，牙周疾病程度由牙龈指数（GI）和牙槽骨吸收比例（ABL）表示。从牙龈炎症更严重部位采集的龈沟液中，α2-M（结合和游离形式）的总量浓度较低。其浓度随着龈袋深度增加以及采集部位牙槽骨吸收的增加而降低。在牙槽骨吸收更明显（超过15%）的部位，龈沟液中α2-M结合形式（即α2-M：蛋白酶复合物的存在）的浓度较低或不存在。我们的结果支持这样的假设，即蛋白酶活性失衡会损害牙周组织。不仅蛋白水解活性，而且抑制活性也发生改变，且与疾病严重程度相对应。
