Condacci I, Cimasoni G, Ahmad-Zadeh C
Infect Immun. 1982 Apr;36(1):66-71. doi: 10.1128/iai.36.1.66-71.1982.
The concentration of alpha 2-macroglobulin (alpha 2-M) in human gingival sulci has been investigated in two studies: first, in gingival washings during a 21-day period of experimental gingivitis in eight human volunteers and, second, in crevicular fluid collected with filter paper strips before and after initial periodontal therapy in 11 patients. The concentration of total alpha 2-M was found to increase in the washings of the volunteers throughout the period of experimental gingivitis. In the group of patients receiving periodontal therapy, the absolute amount of alpha 2-M in the fluid showed a significant decrease after therapy. The gingival index of inflammation and the crevicular fluid flow also decreased significantly. The specific content of the inhibitor (micrograms of alpha 2-M per mg of fluid per min), however, was found to increase in the fluid with decreasing inflammation. As detected by crossed immunoelectrophoresis, the fluid collected in these patients before therapy, in the presence of severe inflammation, invariably showed peaks of both free and complexed alpha 2-M. In contrast, the fluid collected from the same sites after healing of the inflammation contained no detectable free alpha 2-M.
两项研究对人牙龈沟液中α2-巨球蛋白（α2-M）的浓度进行了调查：其一，对8名人类志愿者在为期21天的实验性牙龈炎期间的牙龈冲洗液进行研究；其二，对11名患者在初始牙周治疗前后用滤纸收集的龈沟液进行研究。结果发现，在整个实验性牙龈炎期间，志愿者冲洗液中总α2-M的浓度有所增加。在接受牙周治疗的患者组中，治疗后龈沟液中α2-M的绝对量显著下降。牙龈炎症指数和龈沟液流量也显著降低。然而，随着炎症减轻，龈沟液中抑制剂的特定含量（每分钟每毫克液体中α2-M的微克数）却有所增加。通过交叉免疫电泳检测发现，在这些患者治疗前，炎症严重时，所收集的龈沟液中总是同时出现游离和复合α2-M的峰值。相比之下，炎症愈合后从相同部位收集的龈沟液中未检测到游离α2-M。
