Institute of Food and Agricultural Science Citrus Research and Education Center, University of Florida, 700 Experiment Station Road, 33850, Lake Alfred, Florida.
J Chem Ecol. 1985 Aug;11(8):979-88. doi: 10.1007/BF01020668.
Phytotoxic compounds obtained from methanol extracts of field-grown lantana (Lantana camara L.) were quantified in terms of their inhibition of ryegrass (Lolium multiflorum Lam.) seed germination and/or seedling growth. Subsequent partition of the aqueous fraction (derived from drying the MeOH extract in vacuo and redissolving in distilled water) at various pHs with solvents of differing polarity indicated the phytotoxic compounds were both polar and slightly acidic. Thin-layer Chromatographic separation of the acidic butanol fraction in a solvent mixture of butanol, acetic acid, water (4∶1∶5, by volume) yielded an active fraction with anR f value of 0.89-1.0 that was inhibitory to both root and shoot growth of ryegrass. Two other fractions withR f values of 0.04-0.23 and 0.41-0.57 were inhibitory only to root but not shoot growth.
从田间生长的马缨丹（Lantana camara L.）甲醇提取物中获得的植物毒性化合物，根据其对黑麦草（Lolium multiflorum Lam.）种子萌发和/或幼苗生长的抑制作用进行了定量分析。随后，用不同极性的溶剂在不同 pH 值下对水相（通过真空干燥 MeOH 提取物并重新溶解在蒸馏水中获得）进行分配，表明这些植物毒性化合物既具有极性又略带酸性。在正丁醇、乙酸、水（体积比为 4∶1∶5）的混合溶剂中对酸性丁醇部分进行薄层色谱分离，得到一个活性馏分，其R f 值为 0.89-1.0，对黑麦草根和芽的生长均有抑制作用。另外两个R f 值分别为 0.04-0.23 和 0.41-0.57 的馏分仅对根而不对芽的生长有抑制作用。
