Division of Hematology and Cytogenetics, The Jewish Hospital and Medical Center, Brooklyn, New York, USA.
Theor Appl Genet. 1979 Nov;54(6):277-82. doi: 10.1007/BF00281210.
The nuclear cycle among several diverse genetic stocks of Zea mays root meristem cells was compared and it was found that there were no significant differences among the nuclear cycle durations and its component phases. The durations of various periods of their mitotic cycles were studied by autoradiography of cells pulse-labelled with tritiated thymidine (3H-TdR). The total nuclear cycle was 10 to 11.5 hours and mitosis was 0.81 to 1.34 hours at 25°C. The S period is the longest interval (50% of the total time) of the nuclear cycle; of the rest of the cycle, G2 is longer than G1 or mitosis among all stocks. The constancy of the nuclear cycle among several stocks was adduced as evidence for strict genetic control of the cycle. Furthermore, it is demonstrated the DNA synthesis period is not dependent upon the amount of DNA present.
比较了几种不同遗传品系的玉米根分生组织细胞的核周期，发现核周期及其各组成阶段的持续时间没有显著差异。通过氚标记胸腺嘧啶核苷（3H-TdR）脉冲标记细胞的放射自显影研究了它们有丝分裂周期各个时期的持续时间。在 25°C 时，总核周期为 10 至 11.5 小时，有丝分裂为 0.81 至 1.34 小时。S 期是核周期中最长的间隔（占总时间的 50%）；在其余的周期中，所有品系的 G2 期都长于 G1 期或有丝分裂期。几个品系之间核周期的恒定性被作为周期受到严格遗传控制的证据。此外，还证明了 DNA 合成期不依赖于存在的 DNA 量。
