Laboratoire de Biologie Végétale, Université d'Orléans, F.45046, Orléans Cedex, France.
Plant Mol Biol. 1984 Nov;3(6):419-29. doi: 10.1007/BF00033390.
A cell-free translation system was programmed with total, poly(A), non poly(A) and polysomal RNAs from male and female flowers of this plant with separated sexes. The peptide patterns obtained reflected differences in corresponding translatable RNAs. In total RNA products, three peptides were specific for males, two for females. One of the two male-specific polypeptides of high molecular weight was obtained from poly(A) RNAs and a female-specific one from non poly(A) RNAs. Differences between peptides common to both sexes reflected different concentrations of corresponding messengers. Similar results were obtained with polysomal RNAs. The male-specific RNAs were depending on high endogenous auxin concentrations while the female on active cytokinins. Cytokinin feminization of males induced the female-specific RNAs showing cytokinin action at pretranslational stages. Phytohormone roles are discussed.
用来自雌雄异株植物的雄性和雌性花的总 RNA、多聚 A RNA、非多聚 A RNA 和多核糖体 RNA 对无细胞翻译系统进行了编程。所获得的肽图谱反映了相应可翻译 RNA 的差异。在总 RNA 产物中，有三种肽特异性存在于雄性中，两种特异性存在于雌性中。两种高分子量的雄性特异性多肽之一来自多聚 A RNA，一种雌性特异性多肽来自非多聚 A RNA。在两性共同的肽之间的差异反映了相应信使的不同浓度。多核糖体 RNA 也得到了类似的结果。雄性特异性 RNA 取决于内源生长素的高浓度，而雌性 RNA 则取决于有活性的细胞分裂素。细胞分裂素对雄性的雌性化诱导了特异性 RNA 的出现，表明细胞分裂素在翻译前阶段发挥作用。讨论了植物激素的作用。
