Hamdi S, Teller G, Louis J P
Laboratoire de Biologie végétale, Université d'Orléans 45067 Orléans Cedex 2 France.
Plant Physiol. 1987 Oct;85(2):393-9. doi: 10.1104/pp.85.2.393.
In Mercurialis annua L. (2n = 16) genes for sex determination are considered as major regulator genes controlling stamen and ovary development and sexual phenotypes. After stamen induction, sterility determinants control sporogenous tissue and pollen formation. Moreover, exogenous auxins are able to induce male flowers on female plants. In order to verify if sex and sterility genes have an effect on indole-3-acetic acid (IAA) contents of these plants, various wild or genetically constructed strains were assayed. The IAA levels of their apices were determined by HPLC followed by gas chromatography, selected ion monitoring, mass spectrometry. Results show that high auxin levels are linked to male phenotypes. The genes inducing maleness and the determinants of restored male fertility appear to control and modulate the IAA content. Close correspondence between the number of these dominant genes and IAA levels was established. A final hypothesis about the control of sexual specialization by phytohormones induced by the presence of these genes is discussed.
在一年生山靛（Mercurialis annua L.，2n = 16）中，性别决定基因被认为是控制雄蕊和子房发育以及性别表型的主要调控基因。在雄蕊诱导后，不育决定因素控制孢子发生组织和花粉形成。此外，外源生长素能够在雌株上诱导雄花。为了验证性别和不育基因是否对这些植物的吲哚 - 3 - 乙酸（IAA）含量有影响，对各种野生或基因构建菌株进行了测定。通过高效液相色谱（HPLC），随后进行气相色谱、选择离子监测和质谱分析来测定其顶端的IAA水平。结果表明，高生长素水平与雄性表型相关。诱导雄性的基因和恢复雄性育性的决定因素似乎控制和调节IAA含量。这些显性基因的数量与IAA水平之间建立了密切的对应关系。讨论了关于由这些基因的存在诱导的植物激素控制性别特化的最终假设。
