通过酶联免疫吸附测定法（ELISA）评估C3d部分上新决定簇来测量C3转化。
Measurement of C3 conversion by ELISA estimation of neo-determinants on the C3d moiety.
作者信息
Holmskov-Nielsen U, Jensenius J C, Teisner B, Erb K
出版信息
J Immunol Methods. 1986 Nov 20;94(1-2):1-6. doi: 10.1016/0022-1759(86)90207-3.
An ELISA assay estimating neo-determinants on the C3d moiety is described. The C3d neo-determinants were detected by incubating the sample on F(ab')2 anti-C3d-coated plates followed by development with biotin-labelled anti-C3d and enzyme-labelled avidin. The assay was compared with rocket immunoelectrophoresis (IE) and crossed IE. Serum from a factor I deficient patient showed no detectable C3d when analysed by rocket IE, but activation was evident when analysing by crossed IE or ELISA. The results suggest that the neo-determinants detected by ELISA become exposed when C3 is split into C3a and C3b and this interpretation was supported by kinetic analysis of C3 activation by the three methods.
本文描述了一种用于评估C3d部分上新抗原决定簇的ELISA检测方法。通过将样品在包被有F(ab')2抗C3d的平板上孵育，然后用生物素标记的抗C3d和酶标记的抗生物素蛋白进行显色，来检测C3d新抗原决定簇。该检测方法与火箭免疫电泳（IE）和交叉免疫电泳进行了比较。一名因子I缺乏患者的血清在通过火箭免疫电泳分析时未检测到可检测的C3d，但在通过交叉免疫电泳或ELISA分析时激活明显。结果表明，当C3裂解为C3a和C3b时，ELISA检测到的新抗原决定簇会暴露出来，并且这一解释得到了三种方法对C3激活的动力学分析的支持。
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